Laser Velocimetry (L/V) had made great strides in replacing intrusive devices for wind tunnel flow measurements. The weakness of the L/V has not been the L/V itself, but proper size seeding particles having known drag characteristics. For many Langley Wind Tunnel applications commercial polystyrene latex microspheres suspended in ethanol, injected through a fluid nozzle provides excellent seeding but was not used due to the high cost. This paper provides the instructions, procedures, and formulations for producing polystyrene latex monodisperse microspheres of 0.6, 1.0, 1.7, 2.0, and 2.7 micron diameters. These are presently being used at Langley Research Center as L/V seeding particles.